Principles of Pulse Oximetry Technology:
The principle of pulse oximetry
is based on the red and infrared light absorption
characteristics of oxygenated and deoxygenated hemoglobin. Oxygenated
hemoglobin absorbs more infrared light and
allows more red light to pass through. Deoxygenated
(or reduced) hemoglobin absorbs more red
light and allows more infrared light to pass
through. Red light is in the 600-750 nm wavelength light band.
Infrared light is in the 850-1000 nm wavelength light band.
Pulse oximetry uses a light emitter with red and infrared LEDs that shines
through a reasonably translucent site with good blood flow.
Typical adult/pediatric sites are the finger, toe, pinna (top)
or lobe of the ear. Infant sites are the foot or palm of the
hand and the big toe or thumb. Opposite the emitter is a
photodetector that receives the light that passes through the
There are two methods of sending light through the measuring site: transmission and reflectance. In the transmission method, as shown in the figure on the previous page, the emitter and photodetector are opposite of each other with the measuring site in-between. The light can then pass through the site. In the reflectance method, the emitter and photodetector are next to each other on top the measuring site. The light bounces from the emitter to the detector across the site. The transmission method is the most common type used and for this discussion the transmission method will be implied.
After the transmitted red (R) and infrared (IR) signals pass through the measuring site and are received at the
photodetector, the R/IR ratio is calculated. The R/IR is compared to a "look-up" table (made up of empirical formulas) that convert the ratio to an
SpO2 value. Most manufacturers have their own look-up tables based on calibration curves derived from healthy subjects at various
SpO2 levels. Typically a R/IR ratio of 0.5 equates to approximately 100% SpO2, a ratio of 1.0 to approximately 82%
SpO2, while a ratio of 2.0 equates to 0% SpO2.
The major change that occurred from the 8-wavelength Hewlett Packard oximeters of the '70s to the oximeters of today was the inclusion of arterial pulsation to differentiate the light absorption in the measuring site due to skin, tissue and venous blood from that of arterial blood.
At the measuring site there are constant light absorbers that are always present. They are skin, tissue, venous blood, and the arterial blood. However, with each heart beat the heart contracts and there is a surge of arterial blood, which momentarily increases arterial blood volume across the measuring site. This results in more light absorption during the surge. If light signals received at the photodetector are looked at 'as a waveform', there should be peaks with each heartbeat and troughs between heartbeats. If the light absorption at the trough (which should include all the constant absorbers) is subtracted from the light absorption at the peak then, in theory, the resultants are the absorption characteristics due to added volume of blood only; which is arterial. Since peaks occur with each heartbeat or pulse, the term "pulse oximetry" was coined. This solved many problems inherent to oximetry measurements in the past and is the method used today in conventional pulse
Still, conventional pulse oximetry accuracy suffered greatly during motion and low perfusion and made it difficult to depend on when making medical decisions. Arterial blood gas tests have been and continue to be commonly used to supplement or validate pulse oximeter readings. The advent of "Next Generation" pulse oximetry technology has demonstrated significant improvement in the ability to read through motion and low perfusion; thus making pulse oximetry more dependable to base medical decisions on.